Changelog¶
v2.1¶
- move to read-the-docs.org
v2.0¶
- Update for new Kapa kits.
v1.10¶
- Remove Fragmentase info. Change end-repair temp. to 20 C.
v1.9¶
- Update instructions for adapters to indicate when you would do this (September 24, 2012).
v1.8¶
- Add in NEB incubation temps, which are slightly different.
v1.7¶
- Fix incorrect total volumes for mixes.
v1.6¶
- Minor wording changes for clarity. Change template volume to 15 uL for Phusion PCR alternative.
v1.5¶
- Add adapter annealing section.
v1.4¶
- Add step telling users to aspirate ddH2O containing DNA after steps 64 and 84.
v1.3¶
- Remove all mention of using heat block to dry EtOH from samples.
v1.2¶
- Alter PEG solution mix to Fisher’s original; Change ddH2O volumes for each step so we don’t have to add water twice.
v1.1¶
- Change volumes of PEG Solution used during cleanup steps to increase post-shear to 3X and others to 2.2X. Final is 0.9X, which may be a little bit low.